The Protein Production Platform
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 From cDNA to milligram amounts of protein in as little as four weeks.
 Contact us at mwchen@ntu.edu.sg for more information.


About   Working with PPP   Cloning   Large-scale expression   BEVS   Mammalian expression   Expression vectors   FAQ   



Mammalian expression

We have recently started a mammalian transient expression pipeline in late 2017, by using ExPi cell lines (ThermoFisher) and a selection of expression plasmids. Our priority is given to secreted proteins, cell-surface antigens, antibodies, membrane proteins and multi-subunit complexes. Please contact us for more details.


  • In-silico to in-vitro
    Use the LIMS to create and manage your protein constructs
  • High-throughput cloning
    The platform will clone your protein constructs into E. coli
  • High-throughput protein purification
    Your protein constructs will be purified using IMAC and SEC
  • Purification using affinity chromatography
    All constructs will be fused to a N-terminal cleavable HIS6-tag
  • Expression in insect cells for difficult proteins
    We are currently implementing high-throughput cloning using baculovirus
  • Detergent screening
    Transmembrane proteins will be screened for optimal detergent conditions
  • Multiple quality control steps
    All protein batches will be verified using mass spectrometry and/or sequencing
  • We deliver!
    Receive plasmids, expression strain glycerol stocks and protein batches