The Protein Production Platform
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 From cDNA to milligram amounts of protein in as little as four weeks.
 Contact us at mwchen@ntu.edu.sg for more information.


About   Working with PPP   Cloning   Large-scale expression   BEVS   Mammalian expression   Expression vectors   FAQ   



Expression plasmids for E. coli Cell lines to match
pNIC28-Bsa4 Cleavable N-terminal His-tag. BL21(DE3) Rosetta T1R
C41 (DE3)
BL21(DE3) pFH255
BL21(DE3) pFH256
pNIC-GST Separately cleavable GST fusion in front of cleavable His-tag. BL21(DE3) Rosetta T1R
C41 (DE3)
BL21(DE3) pFH255
BL21(DE3) pFH256
pNIC-CH2 C-terminal His-tag. BL21(DE3) Rosetta T1R
C41 (DE3)
BL21(DE3) pFH255
BL21(DE3) pFH256
pNIC-CTHF Cleavable C-terminal His-FLAG tag. BL21(DE3) Rosetta T1R
C41 (DE3)
BL21(DE3) pFH255
BL21(DE3) pFH256
pNIC-Bio3 Cleavable N-terminal His-tag, C-terminal biotinylation site. BL21(DE3) pCDF-BIRA
pSUMO-LIC Cleavable N-terminal His-SUMO tag. BL21(DE3) Rosetta T1R
C41 (DE3)
BL21(DE3) pFH255
BL21(DE3) pFH256
pNH-TrxT Cleavable N-terminal His-thioredoxin tag. BL21(DE3) Rosetta T1R
C41 (DE3)
BL21(DE3) pFH255
BL21(DE3) pFH256
pCDF-bla-myc Cleavable C-terminal c-myc tag. AmpR; for co-expression with pNIC/pET-based KanR vectors. BL21(DE3) Rosetta T1R
C41 (DE3)
BL21(DE3) pFH255
BL21(DE3) pFH256




Expression plasmids for insect cells Cell lines to match
pFB-LIC-Bse Cleavable N-terminal His-tag. Sf9
High Five
pFB-Sec-NH Cleavable N-terminal His-tag for secreted proteins. Sf9
High Five
pFB-LIC-SUMOStar Cleavable N-terminal His-SUMOStar tag. Sf9
High Five
pFB-Sec-SUMOStar Cleavable N-terminal His-SUMOStar tag; with gp64 signal sequence for secreted proteins. Sf9
High Five




Expression plasmids for mammalian cells Cell lines to match
pCPR0001 Cleavable N-terminal His-2xStrep tag for intracellular expression. ExPi293
HEK293E
pCPR0052 Cleavable C-terminal His-tag for intracellular expression, or secretion if secretion signal included. ExPi293
HEK293E
pHLSec Non-cleavable C-terminal His-tag; with chicken PTPRS signal sequence for secretion. ExPi293
HEK293E